Welcome to the research group "Dynamic Cell Imaging". The term “Cell Imaging” summarises a multitude of methods that are frequently applied in life science. Within this growing field, we routinely apply quantitative fluorescence microscopy and confocal laser scanning microscopy of living cells and aim at the improvement of the in vivo analysis of protein-protein interactions by Förster resonance energy transfer (FRET).
We are interested in the plant endomembrane system and the vacuolar proton translocating ATPase (V-ATPase). Main subjects are the ER-export of vacuolar proteins and the assembly, regulation and targeting of the V-ATPase complex. These topics are addressed by state of the art imaging techniques, but also by molecular biology and biochemical methods for complex isolation and analysis of its subunit composition and regulation. In silico analysis of protein interaction networks contributes to our understanding of V-ATPase regulation and the plasticity of the secretory pathway.
Provenzano, S., Li, Y., Bliek, M., Spelt, C., Appelhagen, I., Machado de Faria, L., Verweij, W., Schubert, A., Sagasser, M., Seidel, T., Weisshaar, B., Koes, R., Quattrochio, F. (2016) Evolution of tonoplast P-ATPase transporters involved in vacuolar hyperacidification. New Phytologist 211(3): 1092-1107
Appelhagen, I., Nordholt, N., Seidel, T., Spelt, K., Quattrocchio, F., Koes, R., Sagasser, M., Weisshaar, B. (2015). Transparent Testa 13 is a tonoplast vacuolar P3A-ATPase required for vacuolar deposition of proanthocyanidins in Arabidopsis thaliana seeds. Plant Journal 82(5): 840-849